Project Area Z

Project Z01

Chemical analyses, isolation, and synthesis of antibiotics

All research projects within the TRR address, in the cellular context, various aspects of antibiotic modes of actions and mechanisms of biosynthesis. To this end, all research projects rely heavily on natural compounds or derivatives thereof, including labeled probes. This central chemistry project provides the analytical support to characterize the structures of bioactive secondary metabolites, characterize the pattern of natural product complexes, and isolate antibacterial natural products for further studies. The project will also produce the required molecules by organic synthesis or semisynthesis.

Principal Investigators

Project Z02

Advanced fluorescence microscopy

Cellular studies on antibiotic action and biosynthesis need to consider the structural order and topological dynamics of bacteria. To this end, the central project Z02 provides a range of classical and advanced fluorescence microscopy techniques to many research projects within this TRR 261. The spatio-temporal distribution of molecular components, their interactions and their dynamics within bacteria, in mammalian cells, or in model membranes will be characterized and quantified. New techniques allowing high resolution imaging of bacteria as well as of selected components in model membranes will be developed and applied.

Principal Investigators

Project Z03

Omics analysis and bioinformatics

The project supports this TRR with the generation, management, bioinformatics analyses, and interpretation of omics data. Almost all projects require significant amounts of genomics, transcriptomics, proteomics, or metabolomics data, and often more than one data modality in parallel. Standardized procedures using next-generation sequencing and high-resolution mass spectrometry coupled to liquid chromatography are the key methods to generate these data. A centralized data management infrastructure ensures reliable data storage, accessibility to the data for all project partners, and automated, reproducible processing of the data, all in close collaboration with the individual projects.

Principal Investigators

Project Z04

Central administration, coordination, and future development of the Collaborative Research Center

This central project coordinates the research projects in Bonn and Tübingen and manages all organizational tasks of the TRR 261 ANTIBIOTIC CellMAP to promote and further develop interactions and innovative research within the consortium. This involves governance and financial administration including project-specific and global funds, organization of central events and seminars of the TRR, coordination of measures for early career support and to promote gender equality, public outreach, and future development.

Principal Investigator