Project Area Z

Cellular Mechanisms

Enabling technologies and central administration

Projects included in Project Area Z will support the consortium by key technologies extensively required by a broad range of projects as well as central administrative tasks. Although the research questions asked and tackled are primarily biology-driven, a strong chemistry support is indispensable in TRR 261, as antibacterial agents are our central study subject. Most of these agents are not commercially available and must be prepared within the consortium. Further support is provided by chemical analytics and natural product purification. Novel fluorescence microscopy techniques based on super-resolution, single molecule tracking, and time-lapse measurements are provided to allow unprecedented insights into the dynamics and organization of prokaryotic cells. An integral 'omics' project combines genomics, transcriptomics, proteomics, and metabolomics to analyze bacterial adaptations and responses to the production of or treatment with antibiotics. This is complemented by bioinformatic support and a state-of-the-art data management infrastructure.


Project Z01

Chemical analyses, isolation, and synthesis of antibiotics

All research projects within the TRR address, in the cellular context, various aspects of antibiotic modes of actions and mechanisms of biosynthesis. To this end, all research projects rely heavily on natural compounds or derivatives thereof, including labeled probes. This central chemistry project provides the analytical support to characterize the structures of bioactive secondary metabolites, characterize the pattern of natural product complexes, and isolate antibacterial natural products for further studies. The project will also produce the required molecules by organic synthesis or semisynthesis.

Principal Investigators
Prof. Dr. Stephanie Grond

Institut für Organische Chemie
Eberhard Karls Universität Tübingen
Auf der Morgenstelle 18 A, 72076 Tübingen
+49 (0)7071 29-72060

Prof. Dr. Dirk Menche

Kekulé-Institut für Organische Chemie und Biochemie
Rheinische Friedrich-Wilhelms-Universität Bonn
Gerhard-Domagk-Str. 1, 53121 Bonn
+49 (0)228 73-2653